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CABRI:ICLC |
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Accession_number |
ICLC HTL01003
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Cell_line_name |
ALL-PO
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Brief_description |
Species: human; Tissue: bone marrow; Tumor: leukemia, acute lymphoblastic
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Description |
Species: human female 4 months old; Tissue: bone marrow; Tumor: leukemia, acute lymphoblastic
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Depositor |
Dr A. Biondi, Clinica Pediatrica, Universita' Milano Bicocca, Monza, Italy
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Bibliographic_reference |
Leuk Res 1997;21:1107-1114 [PMID: 9444945
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Morphology |
lymphoblastic, round, single cells, grown in suspension
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Culture_conditions |
continuous culture; RPMI 1640 + 10% FBS; maintain cultures between 0.5-1 x 10^6 cells/ml; split 1:3-1:6; 37C, 5% CO2
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Viruses |
Search for viruses was not performed
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Release_conditions |
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
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Hazard |
-
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Species_validation |
Validated by HLA-DP beta: confirmed as human by PCR
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Karyology |
t(4;11) translocation
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Freezing_medium |
90% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Comments |
Thaw the cells with medium supplemented with 20% FBS.
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